PHOSPHO1, Recombinant, Human, aa1-267, His-Tag (Phosphoethanolamine/phosphocholine Phosphatase)

Blue Ice

32, 2

Supplied as a liquid in PBS, pH 7.4, 50% glycerol.

Phosphoethanolamine/phosphocholine phosphatase, also known as PHOSPHO1, has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). This protein is involved in the generation of inorganic phosphate for bone mineralization.

Source:
Recombinant protein corresponding to human PHOSPHO1 aa1-267 with a N terminal HIS tag expressed in E. coli (NP_848595).

Molecular Weight:
~32.2kD (291aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.