POLR2H, Recombinant, Human, aa1-150, His-Tag (DNA-directed RNA Polymerases I, II, and III Subunit, HsRPB8, RPABC3, RPB17, RPB8)

Blue Ice

19, 5

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol, 1mM DTT.

POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

Source:
Recombinant protein corresponding to human POLR2H, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~19.5kD (173aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.