RAP2B, Recombinant, Human, aa1-183, His-Tag (Ras-related Protein Rap-2b, RAP2B, Member of RAS Oncogene Family)

Blue Ice

22, 9

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 20% glycerol, 1mM DTT.

Ras-related protein Rap-2b, also known as RAP2B, belongs to a family of RAS-related genes. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. RAP2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

Source:
Recombinant protein corresponding to human RAP2B, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~22.9kD (206aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMREYKVV VLGSGGVGKS ALTVQFVTGS FIEKYDPTIE DFYRKEIEVD SSPSVLEILD TAGTEQFASM RDLYIKNGQG FILVYSLVNQ QSFQDIKPMR DQIIRVKRYE RVPMILVGNK VDLEGEREVS YGEGKALAEE WSCPFMETSA KNKASVDELF AEIVRQMNYA AQPNGDEGCC SACVIL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.