DNAL1, Recombinant, Human, aa1-190, His-Tag (Dynein Light Chain 1, Axonemal, C14orf168, CILD16)

Blue Ice

23, 9

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

DNAL1 is an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. DNAL1 is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.

Source:
Recombinant protein corresponding to aa1-190 from human DNAL1 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~23.9kD (213aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMAKATTI KEALARWEEK TGQRPSEAKE IKLYAQIPPI EKMDASLSML ANCEKLSLST NCIEKIANLN GLKNLRILSL GRNNIKNLNG LEAVGDTLEE LWISYNFIEK LKGIHIMKKL KILYMSNNLV KDWAEFVKLA ELPCLEDLVF VGNPLEEKHS AENNWIEEAT KRVPKLKKLD GTPVIKGDEE EDN

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.