Ro/SS-A Antigen, Recombinant, Human (SSA1, Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

Molecular Biology / MB-Disease Markers

-20°C

Supplied as a lyophilized powder.

Serum amyloid A proteins (SAA) represents a family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122aa nonglycosylated polypeptide, which contains an 18aa N-terminal sequence. Recombinant human apo-SAA1 is an 11.7kD protein containing 105aa.

Source:
Recombinant protein corresponding to human apo-SAA1, expressed in E. coli.

Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 10-10ng/ml.

Endotoxin Level:
<0.1ng/ug (1EU/ug)

AA Sequence:
MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.