CTDSPL, Recombinant, Human, aa82-265, His-Tag (CTD small phosphatase-like protein)

Blue Ice

23, 9

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 40% glycerol.

CTDSPL, also known as CTD small phosphatase-like protein, preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A.

Source:
Recombinant protein corresponding to aa82-265 of human CTDSPL, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~23.9kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLK RPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSK VIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.