DKK3, Recombinant, Human, aa22-350, His-Tag (Dickkopf-related protein 3 precursor)

Blue Ice

38, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1M urea, 1mM DTT, 10% glycerol.

Dickkopf-related protein 3, also known as DKK3, is a member of the DKK protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. DKK3 is a 350aa secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12aa linker region. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Source:
Recombinant protein corresponding to aa22-350 of human DKK3, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~38.8kD

Amino Acid Sequence:
MGSSHHHYHH SSGLVPRGSH MGSMAPAPTA TSAPVKPGPA LSYPQEEATL NEMFREVEEL MEDTQHKLRS AVEEMEAEEA AAKASSEVNL ANLPPSYHNE TNTDTKVGNN TIHVHREIHK ITNNQTGQMV FSETVITSVG DEEGRRSHEC IIDEDCGPSM YCQFASFQYT CQPCRGQRML CTRDSECCGD QLCVWGHCTK MATRGSNGTI CDNQRDCQPG LCCAFQRGLL FPVCTPLPVE GELCHDPASR LLDLITWELE PDGALDRCPC ASGLLCQPHS HSLVYVCKPT FVGSRDQDGE ILLPREVPDE YEVGSFMEEV RQELEDLERS LTEEMALREP AAAAAALLGG EEI

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.