FUBP1, Recombinant, Human, aa279-448, His-Tag (Far upstream element (FuSE) binding protein 1)

Blue Ice

20, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

FuBP1 is a ssDNA binding protein that activates the far upstream element (FuSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FuSE by FuBP occurs through single-strand binding of FuBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Source:
Recombinant protein corresponding to aa279-448 of human FuBP1, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~20.8kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMDVPIP RFAVGIVIGR NGEMIKKIQN DAGVRIQFKP DDGTTPERIA QITGPPDRCQ HAAEIITDLL RSVQAGNPGG PGPGGRGRGR GQGNWNMGPP GGLQEFNFIV PTGKTGLIIG KGGETIKSIS QQSGARIELQ RNPPPNADPN MKLFTIRGTP QQIDYARQLI EEKIG

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.