SNRPA1, Recombinant, Human, aa1-255, His-Tag (u2 small nuclear ribonucleoprotein A)

Blue Ice

30, 5

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 0.1mM PMSF, 30% glycerol.

SNRPA1, also known as u2 small nuclear ribonucleoprotein A, is a component of the u2 snRNP that forms a complex with u2 snRNP B (u2B). Together, u2 snRNP A and u2 snRNP B form a complex that binds to the u2 snRNA hairpin IV. The configuration of this u2 snRNP A/u2 snRNP B dimer and the subtle variations of a few key residues regulate the snRNP-RNA-binding specificity. u2 snRNP A is a 255aa protein, and two isoforms exist as a result of alternative splicing events.

Source:
Recombinant protein corresponding to aa1-255 of human SNRPA1, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~30.5kD

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT VTNGS

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.