EIF3K, Recombinant, Human, aa1-218, His-Tag (Eukaryotic translation initiation factor 3 Subunit K, ARG134, EIF3-p28, EIF3S12, HSPC029, M9, MSTP001, PLAC-24, PLAC24, PRO1474, PTD001)

Blue Ice

27, 2

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.

EIF3K, also known as eukaryotic translation initiation factor 3 subunit K, belongs to the eIF3 subunit K family. It is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. This protein is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. It is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4). Recombinant human EIF3K protein, fused to His-tag at N-terminal, was expressed in E. coli and purified by using conventional chromatography.

Source:
Recombinant protein corresponding to aa1-218 from human EIF3K, fused to His-tag at N-terminal, expressed in E. coli.

Molecular Weight:
~27.2kD (238aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE MLGDLSDSQL KVWMSKYGWS ADESGQIFIC SQEESIKPKN IVEKIDFDSV SSIMASSQ

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.