MGMT, Recombinant, Human, Western Blot Positive Control (O6-methylguanine-DNA methyltransferase)

Blue Ice

25

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate , lethal cross-links at the O6-alkylguanine position in DNA. MGMT removes alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to its cysteine residue, thereby irreversibly inactivating MGMT. Inactivated MGMT is rapidly degraded. Therefore, MGMT levels serves as an indicator of DNA damage. Tumors with high level of MGMT are likely to be drug resistant and low or no MGMT may make the tumor more responsive to chemotherapy. In normal cells, Abnormally low level of MGMT may make normal cells/tissue more susceptible to tumor promoting/alkylating agents.

Source:
Recombinant human MGMT (aa207), expressed in E. coli.

Molecular Weight:
~25kD

Applications:
Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 10ul/lane.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.