Phosphatase, Alkaline, Calf Intestine (1,500-2,500u/mg)

Molecular Biology / MB-Enzymes, Phosphatase

-20°C

Supplied as a lyophilized powder.

Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. This is a cost effective conjugation grade used in antibody preparation supplied as a lyophilized powder.

Activity:
1, 500-2, 500 units/mg protein

Unit Definition:
The amount of enzyme which liberates 1umole of p-nitrophenol from p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in diethanolamine buffer.

Quality Control:
SDS-PAGE
No contaminating RNase, DNase or Proteases detected

Activities:
PDE ≤0.0004%
Adenosine Deaminase ≤0.004%
A-5-MP Deaminase ≤0.004%

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Country of Origin:
Prepared from US origin starting material and collected in USDA approved facilities.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with distilled water or sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.