PHPT1, Recombinant, Human (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase 1, Protein Janus-A Homolog, PHP14, CGI-202, HSPC141)

Blue Ice

15, 9

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 10% glycerol.

PHPT1 is a 125aa enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.

Source:
Recombinant corresponding to aa1-125 of human PHPT1 protein, fused to His-tag at N-terminus, expressed in E.coli.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

Molecular Weight: 15.9kD (145aa) confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.