Reverse Transcriptase, M-MLV, Low RNase H

Blue Ice

Supplied as a liquid in (5x) 250mM Tris-HCl, pH 8.3, 375mM KCl, 15mM MgCl2, 50mM DTT.

Reverse Transcriptase is a new engineered version of M-MLV reverse transcriptase with minimum RNase H activity and maximum thermal stability. The enzyme is purified to near homogeneity to ensure high thermal stability, high specificity, high fidelity, high yield and more full length cDNA synthesis that the premium reverse transcriptase provides. The optimal fist-strand cDNA synthesis temperature for this enzyme is 55oC, and it has a broad working temperature range from 37oC to 60oC, with cDNA product size from 100bp-12Kb.

Source:
E.coli

Unit Definition:
One unit of the enzyme incorporates I nmole of dTTP into acid-precipitable material in 10
minutes at 37°C using poly (A): oligo (dT)25 as template-primer.

Quality Control:
This enzyme has passed the quality control assays: SDS-PAGE analysis for purity, functional absence of endonuclease/nickase activities, functional absence of exonuclease activities, functional absence of protease activity.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.