Tissue, Total Protein, Human Adult Normal, Brain HisTek™

Biologicals / Biologicals-Tissues-Total Protein, Human

-70°C

Supplied as a liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature.

Preparation:
Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70°C.

Applications:
Suitable for Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction and Tissue-Specific Expression Identification. Other applications not tested.

Recommended Dilution:
Western Blot: 10-20ug/lane
Optimal dilutions to be determined by researcher.

Quality Control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70°C. Aliquots are stable for 6 months after receipt.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.