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Recombinant Human FGF-2/FGFb Protein

ArtNr PKSH032438-100ug
Hersteller Elabscience
Menge 100 ug
Quantity options 10 ug 50 ug 100 ug 20 ug
Kategorie
Typ Proteins
Format Lyophilized Powder
Applikationen Cell Culture
Specific against Human (Homo sapiens)
Host E.coli
Konjugat/Tag HIS
Purity > 98 % as determined by reducing SDS-PAGE.
Sequence Ala 135-Ser 288
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Basic fibroblast growth factor, Bfgf, FGF-2, FGF2, FGFB, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Category
Recombinant Proteins / Cytokines
Manufacturer - Conjugate / Tag
N-His
Shipping Temperature
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
18.1 kDa
Observed Molecular Weight
17 kDa
Manufacturer - Research Areas
Signal Transduction, Cardiovascular, Neuroscience, Cancer, Developmental Biology, Stem cells
Background
Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.
Abbreviation
FGF-2; FGFb
Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method.
Reconstitution
Please refer to the printed manual for detailed information.
Formulation
Lyophilized from sterile PBS with 0.01% sarkosyl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FGF-2 is approximately >5 x 105 IU/mg.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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