Anti-Autophagy protein 5 ATG5 Antibody

Primary Antibodies

22048 MW

ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

ATG5 autophagy related 5 homolog (S. cerevisiae)

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

ATG5 autophagy related 5 homolog (S. cerevisiae)

Secreted.

Expressed in bone marrow and testis and
neutrophils.

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.