Mouse Complement C3 Protein, His Tag (MALS verified)

Protein

RT

186.1 kDa

C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Complement C3

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 186.1 kDa. The protein migrates as 70 kDa, 100-120 kDa and 160-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

PBS, pH7.4

trehalose