Anti-Tyrosine Hydroxylase (TYH) Antibody

Primary Antibody

Tyrosine Hydroxylase (TH)

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

200 µg/mL

Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Regular vials contain 1000 µ, l of this antibody mixture, Sampler vials contain 200 µ, l of this mixture.

We produce our Anti-Tyrosine Hydroxylase (TH) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

AB_10013440

1:1000-1:2000

1:1000-1:2000

Human, Mouse, Rat

12 months from receipt of product

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101, NCBI) and mouse (P24529, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.