Recombinant Human NMDA 2B

GRIN2B

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

Hybrid peptides (AA: Arg27-Asp557, Tyr579-Val634, Gln656-Asn817)

Human embryonic kidney 293 (HEK293) cells

Determined by BCA protein assay

N-terminal his-tag

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼85 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼100 kDa) was glycosylated.Lane 1, protein standard ladder (kDa).Lane 2, untreated protein.Lane 3, treated protein with deglycosylation enzymes under native conditions.Lane 4, treated protein with deglycosylation enzymes under denature conditions.Lane 5, deglycosylation enzymes only without target proteins.

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.