Manufacturer |
Raybiotech
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
Human |
Format |
Lyophilized powder |
Applications |
WB, IP, ELISA, Dot |
Amount |
50 ug |
Host |
Hamster - Armenian |
Item no. |
230-30132-50 |
Conjugate/Tag |
His |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Gene Symbols |
GRIN2B |
Application Information |
Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
Storage/Stability |
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS |
Expressed Region |
Hybrid peptides (AA: Arg27-Asp557, Tyr579-Val634, Gln656-Asn817) |
Protein Name & Synonyms |
GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, N-methyl-D-aspartate receptor subunit 3, NR3, Hnr3. |
Expression System |
Human embryonic kidney 293 (HEK293) cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Determined by BCA protein assay |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
Conjugation/Tag Notes |
N-terminal his-tag |
Molecular Weight (kDa) |
Recombinant protein product has a calculated molecular mass of 85 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼85 kDa band. See deglycosylation analysis image below. |
SDS-PAGE Image |
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (¼85 kDa), thus indicating that the untreated recombinant protein (Lane 2, ¼100 kDa) was glycosylated.Lane 1, protein standard ladder (kDa).Lane 2, untreated protein.Lane 3, treated protein with deglycosylation enzymes under native conditions.Lane 4, treated protein with deglycosylation enzymes under denature conditions.Lane 5, deglycosylation enzymes only without target proteins. |
Shipping |
Ice packs |
Custom Labeling Request |
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.