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Recombinant Porcine (pig) IL-13 Protein, HEK293

Recombinant Porcine (pig) IL-13 Protein, HEK293

ArtNr	230-30134-100
Hersteller	Raybiotech
Menge	100 ug
Quantity options	10 ug 100 ug 50 ug
Kategorie	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Typ	Proteins Recombinant
Format	Lyophilized
Applikationen	WB, IP, ELISA, Dot
Specific against	Wild Boar
Host	HEK293 cells
Konjugat/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Animal Proteins
Manufacturer - Conjugate / Tag	N-terminal his-tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of 12 kDa. Due to the abundant glycosylation, it migrates as approximately 25-35 kDa extra protein bands (beside 12 kDa band) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 12 kDa band. See deglycosylation analysis in SDS-PAGE image.
Description	Recombinant Sus scrofa IL-13 protein, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	IL13
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region	Gly21-Gly131
Protein Name & Synonyms	Interleukin-13, IL-13, Bronchial Hyperresponsiveness-1 (Bronchial Asthma), Allergic Rhinitis, P600, NC30.
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by BCA protein assay
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein under reducing conditions.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.Lane 5: deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.