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Recombinant Human ROBO1 Protein, N-terminal, Biotinylated

Recombinant Human ROBO1 Protein, N-terminal, Biotinylated

ArtNr	230-30173B-10
Hersteller	Raybiotech
Menge	10 ug
Quantity options	10 ug 100 ug 200 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Typ	Proteins Recombinant
Format	Lyophilized
Applikationen	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Konjugat/Tag	Biotin, HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of ∼95kDa. Due to the abundant glycosylation, it migrates as approximately ∼110 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼95 kDa band. See deglycosylation analysis image below.
Description	Biotin-conjugated recombinant human ROBO1 protein, N-terminal region with a C-terminal His-tag, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	ROBO1
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Ser20-Pro861
Protein Name & Synonyms	Roundabout Guidance Receptor 1, Deleted In U Twenty Twenty, DUTT1, Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila), Roundabout (Axon Guidance Receptor, Drosophila) Homolog 1, Roundabout, Axon Guidance Receptor, Homolog 1, H-Robo-1, SAX3
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by BCA protein assay
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Endotoxin Level	< 0.5 EU per ug of the protein as determined by the LAL method

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.