Recombinant Human Superoxide Dismutase 1 (SOD1) Protein

Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

N-terminal his-tag

-20°C

Recombinant human Human Superoxide Dismutase 1 (SOD1) Protein with N-terminal His-tag, derived from the transfected human HEK293 cells.

SOD1

Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)

Superoxide dismutase [Cu-Zn], hSod1, Superoxide dismutase 1

His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.

Figure 1.Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼70 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼80 kDa) was glycosylated.Lane 1: Protein standard ladder (kDa)Lane 2: Untreated proteinLane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under denature conditions.Lane 5: Deglycosylation enzymes only without target proteins.