Recombinant Mouse ACE2 Protein

Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

C-terminal his-tag

-20°C

Recombinant mouse angiotensin-converting enzyme 2 (ACE2) with C-terminal his-tag, derived from the transfected human HEK293 cells.

ACE2

Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)

ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15

Lot specific (see the label on the vial), determined by BCA protein assay

Figure 1.Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼80 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼90 kDa) was glycosylated.Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under denature conditions.Lane 5: Deglycosylation mixture only without target proteins.