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Recombinant SARS-CoV-2 S1 Subunit Protein (RBD), mutant N501Y, Biotinylated

Recombinant SARS-CoV-2 S1 Subunit Protein (RBD), mutant N501Y, Biotinylated

ArtNr	230-30184B-100
Hersteller	Raybiotech
Menge	100 ug
Quantity options	100 ug 50 ug 500 ug
Kategorie	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Typ	Proteins Recombinant
Format	Liquid
Applikationen	WB, IP, ELISA, Dot
Specific against	SARS-CoV-2
Host	HEK293 cells
Konjugat/Tag	Biotin, HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Viral Proteins\|COVID-19\|Proteins\|Spike Protein\|Spike Protein Variants
Manufacturer - Conjugate / Tag	C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of ∼25 kDa. Due to the abundant glycosylation, it migrates as approximately ∼30 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one major ∼25 kDa band. See deglycosylation analysis image below.
Description	Biotin-conjugated recombinant SARS-CoV-2 S1 Subunit Protein (RBD) mutant N501Y with C-terminal His-tag, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	S
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Arg319-Phe541. Amino acid Asn (N501) was mutated to Tyr (Y501).
Protein Name & Synonyms	Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial), determined by BCA protein assay
Endotoxin Level	< 0.5 EU per µg of the protein as determined by the LAL method

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.