Human IL-6 protein

Cytokine

IL-6

Lyophilized Human IL-6 protein should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0.

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-6 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Immobilized Human IL-6 protein at 2 μg/mL (100 μl/well) can bind Human IL-6 receptor, The EC50 of Human IL-6 receptor is 440 ng/mL.

BSF2, HGF, HSF, IFNB2, IL-6, Interleukin-6, Interleukin-6, IL-6