Human TNF-alpha protein

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.

E. coli

Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0.

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TNF-alpha protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The product is shipped at ambient temperature.

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

DIF; TNF-alpha; TNFA; TNFSF2; Tumor necrosis factor; TNF

TNF