Rat TNF-α protein

Cytokine

TNF-α

Lyophilized Rat TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.107% HSA or BSA). Please prevent freeze-thaw cycles.

Tumor necrosis factor α (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Lyophilized from sterile 20 mM tris 50 mM NaCl, pH 8.0.

Endotoxin: <0.1 EU per µg of the protein as determined by the LAL method.

E. coli

The recombinant Rat TNF-α consists of 156 amino acids and has a predicted molecular mass of 17.2 kDa.