Human CD73/NT5E protein, His tag (Animal-Free)

The recombinant human CD73 protein consists of 528 amino acids and predicts a molecular mass of 59 kD.The apparent molecular mass of the human CD73 is approximately 70 kDa in SDS-PAGE under reducing conditions due to glycosylation.

>95% as determined by SDS-PAGE

Endotoxin: < 1 EU per ug of the protein as determined by the LAL method.

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD73 protein in the buffer which we provide not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.

The product is shipped at ambient temperature.

CD73 (ecto-5'-nucleotidase) is a novel immunoinhibitory protein that plays a key role for tumor growth and metastasis. Its main function is to convert extracellular ATP to immunosuppressive adenosine in concert with CD39 in normal tissues to limit excessive immune response. However, tumors take advantage of the CD73-mediated adenosinergic mechanism to protect them from immune attack. In particular, inducible expression of CD73 along with other adenosinergic molecules on both cancer cells and host cells sustains immunosuppressive tumor microenvironment by affecting multiple aspects of the immune response. Owing to its multifaceted capacity to tumor promotion as an emerging immune checkpoint, CD73 is an ideal therapeutic target for cancer treatment especially in combination with conventional therapy and/or other immune checkpoint inhibitors.

5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

CD73/NT5E