Human Annexin V/ANXA5 Protein (AN5-HE005)

ArtNr	AN5-HE005-100UG
Hersteller	KactusBio
Menge	100 ug
Quantity options	100 ug 500 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Apoptosis
Typ	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	E.coli
Konjugat/Tag	Untagged
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula	Supplied as 0.22μm filtered solution in 50mM Tris, 240mM NaCl (pH 8.5).
Sequence	Ala2-Asp320
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Annexin A5,Anchorin CII,Annexin V,Annexin-5,Calphobindin I,CBP-I,Endonexin II,PP4,PAP-I,VAC-alpha,ANX5,ENX2,ANXA5,HEL-S-7,RPRGL3
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	Annexin V/ANXA5
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 35.9 kDa same as Bis-Tris PAGE result.
Description	Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.
Background	Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 50mM Tris, 240mM NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	no

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

AN5-HE005-100UG-datasheet.pdf

AN5-HE005-100UG-safety-datasheet.pdf