Ketohexokinase, 1-298aa, Human, E.coli

Ketohexokinase is an enzyme that catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, leading to consumption of ATP, formation of AMP. This protein initiates first step in the metabolism of dietary fructose and is an important regulator of hepatic glucose metabolism. It is highly found in liver, renal cortex, and small intestine. Its deficiency causes the benign hereditary metabolic disorder essential fructosuria, leading to fructose being excreted in the urine. Recombinant human Ketohexokinase was expressed in E.coli and purified by using conventional chromatography.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Bonthron DT., et al. (2009) J Histochem Cytochem. 57(8):763-74.
• Schermerhorn T., et al. (2009) Res Vet Sci. 87(1):115-7.

>, 90% by SDS - PAGE

MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV