Desmin , human, recombinant

Life Sciences / Peptides and Biochemicals / Research Peptides

-20 C

Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.Description: Desmin Human Recombinant having a calculated molecular mass of 53, 539 Dalton, showing a 55kDa band on SDS-page, pI-5.16.Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.Formulation: Desmin was lyophilized from a 1mg/ml solution containing 30mM Tris-HCl pH 8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.Solubility: It is recommended to reconstitute the lyophilized Desmin in sterile 18MΩ -cm H2O not less than 100µ g/ml, which can then be further diluted to other aqueous solutions.Stability: Lyophilized Desmin although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution Desmin should be stored at 4° C between 2-7 days and for future use below -18° C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.Reconstitution to filaments: After Desmin is dissolved in 9.5M urea buffer (see formulation), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).