Comparison

Desmin , human, recombinant European Partner

Item no. BNTH-PRO-520-0,005mg
Manufacturer BIOSYNTH
Amount 0.005 mg
Category
Type Proteins Recombinant
Specific against other
Host E.coli
Purity Greater than 95.0% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Desmin, DES, CSM1, CSM2, CMD1I, FLJ12025, FLJ39719, FLJ41013, FLJ41793.
Available
Manufacturer - Category
Life Sciences / Peptides and Biochemicals / Research Peptides
Shipping Temperature
No Dry Ice
Storage Conditions
-20 C
Model
Desmin Human Recombinant
Description
Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.Description: Desmin Human Recombinant having a calculated molecular mass of 53, 539 Dalton, showing a 55kDa band on SDS-page, pI-5.16.Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.Formulation: Desmin was lyophilized from a 1mg/ml solution containing 30mM Tris-HCl pH 8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.Solubility: It is recommended to reconstitute the lyophilized Desmin in sterile 18MΩ -cm H2O not less than 100µ g/ml, which can then be further diluted to other aqueous solutions.Stability: Lyophilized Desmin although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution Desmin should be stored at 4° C between 2-7 days and for future use below -18° C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.Reconstitution to filaments: After Desmin is dissolved in 9.5M urea buffer (see formulation), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.005 mg
Available: In stock
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