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Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101)

ArtNr CAL-HE101-100UG
Hersteller KactusBio
Menge 100 ug
Quantity options 100 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Specific against Human (Homo sapiens)
Host E.coli
Konjugat/Tag HIS
Purity > 95% as determined by Bis-Tris PAGE
Formula Lyophilized from 0.22μm filtered solution in 2mM DTT, PBS (pH 7.2). Normally 5% trehalose is added as protectant before lyophilization.
Sequence Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Protein S100-A8,Calgranulin-A,CFAG,MRP-8,S100 calcium-binding protein A8,Protein S100-A9,MRP-14,S100 calcium-binding protein A9
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Recombinant Protein
Manufacturer - Targets
Calprotectin (S100A8&S100A9)
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
Shipped at ambient temperature.
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight
The protein has a predicted MW of 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Bis-Tris PAGE result.
Description
Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus of S100A8.It contains Met1-Glu93(S100A8) and Thr2-Pro114(S100A9).
Background
Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.
Endotoxin
Less than 1 EU per μg by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated
no

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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