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Human CD73/NT5E Protein (CD7-HM273)

ArtNr CD7-HM273-100UG
Hersteller KactusBio
Menge 100 ug
Quantity options 100 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Specific against Human (Homo sapiens)
Host HEK293
Konjugat/Tag C-hFc
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Sequence Trp27-Lys547
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD73,NT5E,NTE,5' nucleotidase (CD73),5'-NT,5-NT,E5NT,EC 3.1.3.5,eN,eNT,NT,NT55'-nucleotidase,NT5,NTE,CALJA
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Recombinant Protein
Manufacturer - Targets
CD73/NT5E
Shipping Temperature
Shipped at ambient temperature.
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight
The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Description
Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFC tag at the C-Terminus.It contains Trp27-Lys547.
Background
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Endotoxin
Less than 1 EU per μg by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated
no

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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