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Mouse CD73/NT5E Protein (CD7-MM173)

ArtNr CD7-MM173-100UG
Hersteller KactusBio
Menge 100 ug
Quantity options 100 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Specific against Mouse (Murine, Mus musculus)
Host HEK293
Konjugat/Tag HIS
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization.
Sequence Trp29-Lys549
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD73,NT5E,NTE,5' nucleotidase (CD73),5'-NT,5-NT,E5NT,EC 3.1.3.5,eN,eNT,NT,NT55'-nucleotidase,NT5,NTE,CALJA
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Recombinant Protein
Manufacturer - Targets
CD73/NT5E
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
Shipped at ambient temperature.
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Description
Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.
Background
CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Endotoxin
Less than 1 EU per μg by the LAL method.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated
no

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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