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Biotinylated Human / Cynomolgus / Rhesus macaque CD28 Protein, His,Avitag™, active dimer (MALS verified) Europäischer Partner

ArtNr CD8-H82E5-25ug
Hersteller ACROBiosystems
Menge 25 ug
Quantity options 200 ug 25 ug
Kategorie
Typ Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens), Monkey (Cynomolgus, Simian), Rhesus macaque
Host HEK293
Konjugat/Tag Biotin, Avi
Purity 90%
Citations (1)Evans E.J., et al., 2005, Nat. Immunol. 6:271-279. (2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46. (3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
NCBI NP_006130.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD28,Tp44
Versandbedingung Raumtemperatur
Lieferbar
Specificity Human / Cynomolgus / Rhesus macaque
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
C-10xHis & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
18.7 kDa
Manufacturer - Format
Powder
Description
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His, Avitag (CD8-H82E5) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
Background
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Molecule
CD28
Exp Region
Asn 19 - Pro 152
Characteristics
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 18.7 kDa. The protein migrates as 33-45 kDa under reducing (R) condition, and 55-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
PBS, pH7.4
Stability
● -20°C to -70°C for 12 months in lyophilized state
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
lieferbar

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