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Recombinant Bovine Beta-1,4-galactosyltransferase 1(B4GALT1),partial

ArtNr CSB-EP002513BO-20
Hersteller Cusabio
Menge 20 ug
Quantity options 1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Kategorie
Typ Proteins Recombinant
Specific against other
Host E.coli
Konjugat/Tag GST
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MKFREPLLGGSAAMPGASLQRACR
Protein Familie Glycosyltransferase 7 family
Citations The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I.Ramasamy V., Ramakrishnan B., Boeggeman E., Qasba P.K.J. Mol. Biol. 331:1065-1076(2003)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
Lieferbar
Manufacturer - Conjugate / Tag
N-terminal GST-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
29.5 kDa
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Relevance
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the Extracellular domain matrix.
Expression Region
1-24aa
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; FUNCTION
Subcellular Location
Isoform Long: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Cell membrane, Single-pass type II membrane protein, Cell surface, Cell projection, filopodium, Note=Found in trans cisternae of Golgi, B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity), SUBCELLULAR LOCATION: Isoform Short: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Note=Found in trans cisternae of Golgi, SUBCELLULAR LOCATION: Processed beta-14-galactosyltransferase 1: Secreted
Gene Names
B4GALT1
Sequence Info
Cytoplasmic Domain
Organism
Bos taurus (Bovine)
Manufacturer - Format
Liquid or Lyophilized powder

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20 ug
Lieferbar: In stock
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