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Mouse LIF Protein, His Tag (MALS verified)

Mouse LIF Protein, His Tag (MALS verified)

ArtNr	LIF-M5227-20ug
Hersteller	ACROBiosystems
Menge	20 ug
Quantity options	500 ug x 2 20 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Solid
Specific against	Mouse (Murine, Mus musculus)
Host	HEK293
Konjugat/Tag	Unconjugated
Purity	90%
Citations	(1) Wenwei Hu, Zhaohui Feng, Angelika K. Teresky1, Arnold J. Levine (November 29, 2007). (2) Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L (2009). PLoS ONE 4 (7): e6343. (3) Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).
NCBI	NP_032527.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	LIF,CDF,DIA,HILDA,MLPLI
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	C-6xHis, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	20.8 kDa
Manufacturer - Format	Powder
Description	Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Background	Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Molecule	LIF
Exp Region	Ser 24 - Phe 203
Characteristics	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.8 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	PBS, pH7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

LIF-M5227-20ug-datasheet.pdf

LIF-M5227-20ug-safety-datasheet.pdf