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Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein (MHC-HM447T)

Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein (MHC-HM447T)

ArtNr	MHC-HM447T-100UG
Hersteller	KactusBio
Menge	100 ug
Quantity options	100 ug 500 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	HEK293
Konjugat/Tag	HIS, Avi
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Sequence	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
NCBI	ALYVDSLFFL
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Lieferbar
Manufacturer - Product Type	Recombinant Protein
Description	Recombinant Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ALYVDSLFFL peptide.
Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Endotoxin	Less than 1 EU per μg by the LAL method.
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Target	HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer
Manufacturer - Tag	C-His-Avi
Stability and Storage	-20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Biotinylated	no

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

MHC-HM447T-100UG-datasheet.pdf

MHC-HM447T-100UG-safety-datasheet.pdf