Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Redox Proteins

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Aldo-keto Reductase1C1, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1, Aldo-keto reductase family 1, member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20 alpha hydroxysteroid dehydrogenase, 2ALPHAHSD, AK1C1, MBAB, Aldo keto reductase family 1 member C1, C9, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRC, DD1, Dihydrodiol dehydrogenase 1, Dihydrodiol dehydrogenase 1/2, H37, HAKRC, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, Trans-1, 2 dihydrobenzene 1, 2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase, ATGP0496-10ug, ATGP0496-20ug, ATGP0496-50ug, ATGP0496-100ug, ATGP0496-250ug, ATGP0496-500ug, ATGP0496-1mg, ATGP0496-10, ATGP0496-20, ATGP0496-50, ATGP0496-100, ATGP0496-250, ATGP0496-500, ATGP0496-1