Recombinant human CDA protein

SDS-PAGE, Enzyme Activity

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100mM NaCl, 40% glycerol

Enzymes & Proteases

Specific activity is > 10, 000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.

CDA, Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase, ATGP0739-10ug, ATGP0739-20ug, ATGP0739-50ug, ATGP0739-100ug, ATGP0739-250ug, ATGP0739-500ug, ATGP0739-1mg, ATGP0739-10, ATGP0739-20, ATGP0739-50, ATGP0739-100, ATGP0739-250, ATGP0739-500, ATGP0739-1