Recombinant human UCH-L5/UCH37 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA

Ubiquitination

Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

UCH-L5, UCHL5, UCH-37, UCH37, UCH L5, UCH 37, Ubiquitin thioesterase L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin carboxyl terminal hydrolase isozyme L5, Ubiquitin C terminal hydrolase UCH37, INO80R, INO80 complex subunit R, CGI-70, CGI70, CGI 70, AD-019, AD019, AD 019, ATGP1069-10ug, ATGP1069-20ug, ATGP1069-50ug, ATGP1069-100ug, ATGP1069-250ug, ATGP1069-500ug, ATGP1069-1mg, ATGP1069-010, ATGP1069-020, ATGP1069-050, ATGP1069-100, ATGP1069-250, ATGP1069-500, ATGP1069-01M