Recombinant human O-fucosyltransferase 1/POFUT1 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

GDP-fucose protein O-fucosyltransferase 1, also known as POuFuT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFuT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFuT1 plays an important role in Notch signaling, as Notch ligands can serve as POFuT1 substrates. Recombinant human POFuT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol

Neuroscience

Human