Recombinant human eIF-3 beta/EIF3I protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E. coli.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Epigenomics (Transcription & Translation)

Human

C3orf68, eIF3-beta, eIF-3-beta, eIF3i, eIF3-p36, EIF3S2, Eukaryotic translation initiation factor 3 subunit 2, Eukaryotic translation initiation factor 3 subunit 2 beta 36kDa, Eukaryotic translation initiation factor 3 subunit I, PRO2242, TGF-beta receptor-interacting protein 1, TRIP1, TRIP-1