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Recombinant E.coli D-lactate dehydrogenase/LDHA protein

ArtNr NKM-ATGP3236-250ug
Hersteller NKMAX
Menge 250 ug
Quantity options 100 ug 10 ug 20 ug 250 ug 500 ug 50 ug
Kategorie
Typ Proteins Recombinant
Format Liquid
Applikationen SDS-PAGE
Specific against Escherichia coli (E.coli)
Host E.coli
Konjugat/Tag HIS
Purity > 95% by SDS-PAGE
Sequence <MGSSHHHHHH SSGLVPRGSH MGSH>MKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP
Citations Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1): 13-22.
Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11): 25026-25037.
NCBI NP_415898.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias NAD-dependent,JW1375,htpH,hslI,hslF,Fermentative D-lactate dehydrogenase,ECK1377
Similar products ldhA, D-lactate dehydrogenase, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10ug, ATGP3236-20ug, ATGP3236-50ug, ATGP3236-100ug, ATGP3236-250ug, ATGP3236-500ug, ATGP3236-1mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1
Lieferbar
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Enzyme Activity
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
39.1 kDa (353aa) confirmed by MALDI-TOF
Description
Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100mM NaCl
Antigen Species
E.coli
Gene Category
Redox Proteins
Domain
1-329aa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only
Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Species of Protein
E.coli
Manufacturer - Search Terms
D-lactate dehydrogenase, ldhA, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10ug, ATGP3236-20ug, ATGP3236-50ug, ATGP3236-100ug, ATGP3236-250ug, ATGP3236-500ug, ATGP3236-1mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 250 ug
Lieferbar: In stock
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