Recombinant mouse Sepiapterin reductase/SPR protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 10% glycerol

Redox Proteins

Mouse