Recombinant SARS-CoV Spike RBD protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Virus

< 1 EU per 1ug of protein (determined by LAL method)

Asp

covid-19, covid19, sars, sars2, Sars-CoV-2 S1, covid, corona, ATGP3966-10ug, ATGP3966-20ug, ATGP3966-50ug, ATGP3966-100ug, ATGP3966-250ug, ATGP3966-500ug, ATGP3966-1mg, ATGP3966-10, ATGP3966-20, ATGP3966-50, ATGP3966-100, ATGP3966-250, ATGP3966-500, ATGP3966-1