Recombinant human NCAM-1/CD56 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

NCAM-1, also known as CD56, is a member of the immunoglobulin superfamily. It specifically binds to neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. This protein is preferentially expressed in NK cells and a subset of T lymphocytes that mediate MHC-unrestricted cell-mediated cytotoxicity. High expression of NCAM-1/CD56 differentiates NK cells as having an activated phenotype. CD56(+high) NK cells mediate heightened effector functions in response to IL-12. During hematopoiesis, NCAM-1/CD56 is the prototypic marker of NK cells, also present on subset of CD4+ T cells and CD8+ T cells. In cell adhesion, it contributes to cell-cell adhesion or cell-matrix adhesion during embryonic development. In anatomic pathology, pathologists make use of it immunohistochemistry to recognize certain tumors. Recombinant human NCAM-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

< 1 EU per 1ug of protein (determined by LAL method)

Human